| 产品编号 | bsm-33034M-HRP |
| 英文名称 | Mouse Anti-Beta tubulin(Loading Control)/HRP Conjugated antibody |
| 中文名称 | 辣根过氧化物酶标记的微管蛋白β tubulin/Tubulin β单克隆抗体（内参抗体） |
| 别 名 | Beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; Beta1 tubulin; Class I beta tubulin; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SWcl.56; TBB5_HUMAN; TUBB 1; TUBB 2; TUBB 5; TUBB; TUBB1; TUBB2; TUBB5; tubulin beta 1 chain; Tubulin beta 2 chain; tubulin beta 5 chain; Tubulin beta chain; Tubulin beta class I; tubulin beta polypeptide; Tubulin beta-5 chain. |
| 规格价格 | 100ul/1200元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 内参抗体 |
| 研究领域 | 细胞骨架 细胞外基质 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 7G3 |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, Monkey, Insect, Yeast) |
| 产品应用 | WB=1:10000-1:50000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Beta tubulin |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Subunit: Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1. Subcellular Location: Cytoplasmic, cytoskeleton. Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Post-translational modifications: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). Similarity: Belongs to the tubulin family. Database links: Entrez Gene: 203068 Human Entrez Gene: 22154 Mouse Omim: 191130 Human SwissProt: P07437 Human SwissProt: P99024 Mouse Unigene: 636480 Human Unigene: 2458 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Human Hela cell lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human Jurkat cell lysates
Lane 7: Human Huvec cell lysates
Primary: Anti-Beta tubulin/HRP (bsm-33034M-HRP) at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
