| 产品编号 | bsm-41511R |
| 英文名称 | SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Recombinant Rabbit mAb |
| 中文名称 | SARS冠状病毒2核衣壳蛋白单克隆抗体（新冠抗原检测原料，可识别奥密克戎） |
| 别 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein; Ormicron mutant; Ormicron Nucleocapsid Protein; Ormicron N Protein. |
| 研究领域 | 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 7B2 |
| 交叉反应 | SARS-CoV-2 WT,SARS-CoV-2 (D614G),SARS-CoV-2 Omicron and series of mutants |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant SARS-CoV-2 Nucleocapsid Protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 产品图片 |
Sample:
Lane 1:SARS-CoV-2 N Protein (WT) at 10ng
Lane 2:SARS-CoV-2 N Protein (Del204 & Del215) at 10ng
Lane 3:SARS-CoV-2 N Protein (R203M & D377Y) at 10ng
Primary: Rabbit Anti- SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46kD
Observed band size:46kD
Sample:
Lane 1:SARS-CoV-2 N Protein (WT) at 10ng
Lane 2:SARS-CoV-2 N Protein (Del204 & Del215) at 2ng
Lane 3:SARS-CoV-2 N Protein (R203M & D377Y) at 2ng
Primary: Rabbit Anti- SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46kD
Observed band size:46kD
Sample:
Lane 1: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng
Lane 2: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng
Lane 3: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng
Lane 4: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng
Lane 5: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng
Lane 6: Recombinant SARS-CoV-2 N Protein(WT) at 10ng
Primary: Rabbit Anti- SARS-CoV-2 N protein (bsm-41511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:45.3 kD
Observed band size:50 kD
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
