| 产品编号 | bsm-33346M |
| 英文名称 | Flag Tag Recombinant Mouse mAb |
| 中文名称 | Flag Tag标签单克隆抗体 |
| 别 名 | DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag. |
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Specific References (3) | bsm-33346M has been referenced in 3 publications.
[IF=11.205] Cong Lan. et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBIOMEDICINE. 2022 Aug;82:104139 WB ; Rat.
[IF=8.077] Kang Kexin. et al. Dishevelled phase separation promotes Wnt signalosome assembly and destruction complex disassembly. J CELL BIOL. 2022 Dec;221(12):e202205069. WB ; Human.
[IF=5.428] Xusheng Du. et al. RIOK3-mediated Akt phosphorylation facilitates synergistic replication of Marek’s disease and reticuloendotheliosis viruses. VIRULENCE. 2022 Jul 06 IF ; Chicken.
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| 产品类型 | 标签抗体 |
| 研究领域 | 细胞生物 |
| 抗体来源 | Mouse |
| 克隆类型 | Recombinant |
| 克 隆 号 | 3D7 |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:10000-100000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Flag Tag |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. |
| 产品图片 |
Sample:
DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P)
Primary: Anti-Flag Tag (bsm-33346M) at 1/5000 ~ 1/100000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa
20 ng Recombinant human TNFSF13B protein (HEK293)，N-hFc-Flag (bs-43132P) per lane probed with Flag Tag monoclonal antibody respectively, unconjugated (bsm-33346M) at 1:50000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
